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Objective To abserve the acute toxicity in mice administered with Wuweizi Ningshen oral liquid.Methods The maximum administration dosage(MAD)of Wuweizi Ningshen oral liquid were determined by administration intragastricly in mice.Wuweizi Ningshen oral liquid was administered for 2 weeks,and the growth condition,body weight growth and index of main viscera were measured.Results The index of growth condition,body weight growth and index of main viscera were in normal ranges.Conclusion It could be concluded that Wuweizi Ningshen oral liquid is safe to be administered in the dose prescribed.
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OBJECTIVE:To prepare compound ofloxacin gel and to establish its quality control method.METHODS:Ofloxacin was used as principal agent to be mixed with dexamethasone sodium phosphate,carbomer 940 was taken as base material,the content of ofloxacin and dexamethasone were determined by HPLC method.RESULTS:The linear detection concentration ranges of ofloxacin and dexamethasone were 20～300?g/ml and 5～50?g/ml,respectively,the average recovery rates of which were (99.8?0.5)%(RSD=0.84%)and (100.6?0.8)%(RSD=0.87%),respectively.CONCLUSION:The preparation is stable in quality,the prepare technique is simple and the quality control is reliable.
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OBJECTIVE:To prepare diclofenac sodium controlled release pellets and investigate the mechanism of re?lease.METHODS:Single-factor method was used to investigate the influence of the composition of solvent system in coating solution,the concentration of coating material,porogenic agent and plastifier on the release rate of drug.RESULTS:When the concentration of coating material and the proportion of water increased,the release rate of pellets was increased;the release rate was further increased with PVP K30 added.CONCLUSION:The pellets belongs to the matrix-coating pellets,and the release mechanism varies with the change of coating thickness.
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OBJECTIVE:To prepare red sage gel for implantation and to establish a method for its quality control.METHODS:With carbamer934as vehicle,2%red sage gel was prepared;a HPLC method for the determination of tanshinone in gel was established.RESULTS:The calibration curve of tanshinoneⅡ A was linear in the concentration range of16.59～33.18ng/ml,Y=9723X—2569(n=5),r=0.9987.CONCLUSION:The preparation of red sage gel was simple,its quality was stable;the method of quality control was rapid and accurate.
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OBJECTIVE:To establish a HPLC method to determine the content of compound tramadol tablets METHODS:Stationary phase:Kromasil C18 column,internal standard:phenacetin,mobile phase:methanol-water-triethlamine(55∶45∶0 2),adjusting pH to 4 2 with acetic acid,flow rate∶0 7ml/min,detecting wavelength∶267nm RESULTS:The linear range of tramadol (TR) was 50～500?g/ml(r=0 9 999,n=5) The linear range of nefopam(NFP) was 50～500?g/ml(r=0 9 999,n=5) The average recovery of TR was 99 69% The average content of TR was 100 71%,RSD=0 76%(n=5) The average recovery of NFP was 99 76% The average content of NFP was 99 64%,RSD=0 62%(n=5) CONCLUSION:The method is accurate,rapid and simple
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OBJECTIVE: To study the antibacterial actions of Qinghou buccal tablets in vivo and in vitro. METHODS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of Qinghou buccal tablets were determined by standard test tube doubling dilution method;And the antibacterial action of which on staphylococcus aureus in mice in vivo were monitored. RESULTS: Qinghou buccal tablets had significant in vitro antibacterial action on staphylococcus aureus, ?-hemolytic streptococcus, ?-hemolytic streptococcus, streptococcus pneumoniae and hemophilus influenza, with MIC and MBC at 0.0 625～0.50g/ml and 0.125～1.0g/ml, respectively,and which had bacteriostatic action on mice that injected i.p. with staphylococcus aureus. CONCLUSION: Qinghou buccal tablets were proved to be of bacteriostatic actions in vitro and in vivo.